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The Use, Maintenance, and Care of pH Electrodes in Fermentor

Post on 2024-11-28

I. Preparation before use

1. Before opening the package, please check if the package is damaged. If the outer packaging is damaged, do not open it further. It is better to immediately contact the transport department and the company, and you can jointly open the package with the representative of the transportation department to inspect the electrode to check if it is damage. It is recommended to take photos.

2. If the outer packaging is intact but the electrode is damaged, please immediately contact the company. Under this circumstance, it is better to send the electrode along with the warranty card, user manual, and original packaging back to the company.

3. Read the user manual carefully before using the electrode. If the membrane of the electrode is covered with silicone, carefully scrape off the silicone with the small knife provided with the electrode before use, in the direction from front to back, to avoid damaging the sensitive membrane. It will better to make sure that all the silicone on the membrane is removed (until the tip of the knife touches the porous ceramic on the membrane).

4. It is better to check if the sensitive membrane of the pH electrode ball is filled with liquid. If there is no liquid or air bubbles, gently shake the electrode to fill the ball with liquid, and make sure that there is not air bubbles in the ball.

5. Before using the electrode, you can soak it in acid buffer solution (pH 4.00) for a few minutes, then soak it in neutral buffer solution (pH 6.86 or 7.00, etc.) for a few minutes. Then the calibration is started.

II. Attention during electrode calibration

1. Please pay attention to using fresh buffer solution during calibration. Do not use the buffer solution if it fades and becomes ineffective.

2. Place the electrode in the buffer solution for 1 minute before proceeding with the subsequent operation.

3. Pay attention to selecting the correct buffer solution series in the transmitter.

4.After the electrode is rinsed, only use the soft paper tissue to absorb the moisture. Do not rub the ph-sensitive membrane.

5.The calibration cycle of the electrode is determined according to the different use environment and accuracy requirements, and the appropriate calibration cycle is determined under the premise of ensuring the accuracy.

III. Daily maintenance of pH electrodes

1. General contamination: Clean the electrode with water, 0.1mol/l NaOH or 0.1mol/l HCl for several minutes.

2. Oil or organic contamination: Clean the electrode with acetone or ethanol for a few seconds.

3. Sulfide contamination (membrane turns black): Treat with 9892 cleaning solution.

4. Protein contamination (membrane turns yellow): Treat with 9891 cleaning solution.

5. Electrode regeneration: Treat with 9895 regeneration solution. Regeneration can only extend the life of the electrode to a limited extent.

6. Casing pressure maintenance: For pressure-sensitive electrodes, pay attention to regularly monitoring the pressure gauge on the casing. In principle, the pressure inside the casing should be 1.5 Bar higher than the pressure in the reaction vessel.

7. When the measured medium is contaminated by sulfide or protein, it should be treated in a timely manner.

IV. Preservation of pH electrodes

1. According to the instructions,the electrode should be placed in suitable liquid when not in use for a long time.

2. The electrode cannot be placed in the dry circumstance for a long time, and it should not be stored with dry medium on its surface. The dry electrode should be activated by placing it in suitable preservation liquid before use.

3. The electrode should not be stored in distilled water.

V. Instructions for using electrode cleaning solution

1. Electrode cleaning solution

Gastric secretin/HCl is used to remove protein contamination (yellowing of the diaphragm). Method of use: Place the head of electrode into the solution, making sure the diaphragm is immersed in the solution (at least 1 hour). Then it is rinsed with distilled water and recalibrated.

2. Diaphragm cleaning solution

Thiourea/HCl is used to clean blackened diaphragms which is caused by sulfur solutions. Method of use:The head of electrode is immersed in the solution (The diaphragm should be immersed in the solution), until the diaphragm is colorless (at least 1 hour), then it is immersed in 3M KCl overnight, after it is rinsed, it can be used after recalibration.

3. Electrode regeneration solution

Hold the electrode with the sensitive membrane downward, and drop the electrode regeneration solution on the sensitive membrane. The electrode is continuously rotated along its axis, it is recommended to be kept for about 3 minutes, then immediately rinse the electrode with deionized water and store it in the preservation liquid specified in the electrode usage instructions for 12 hours. It will be used after it is re-calibrated.